| 质粒名称: | Tet-on circRNA Expression Vector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92351 |
| 存储实验室: | Ling-Ling Chen |
|
载体骨架基本信息 |
|
| 载体名称: | p23-phage |
| 载体出品公司: | -- |
| 载体抗性: | 低氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry and split EGFP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | pTet |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Tet-on circRNA Expression Vector was a gift from Ling-Ling Chen (Addgene plasmid # 92351 ; http://n2t.net/addgene:92351 ; RRID:Addgene_92351) |
| 参考文献部分: | The Biogenesis of Nascent Circular RNAs. Zhang Y, Xue W, Li X, Zhang J, Chen S, Zhang JL, Yang L, Chen LL. Cell Rep. 2016 Apr 19;15(3):611-24. doi: 10.1016/j.celrep.2016.03.058. Epub 2016 Apr 7. 10.1016/j.celrep.2016.03.058 PubMed 27068474 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Tet-on circRNA Expression Vector 10054 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION The vector can ef_ciently produce a circRNA (circmCherry) from a
split EGFP gene after Dox treatment..
ACCESSION .
VERSION .
KEYWORDS Tet-on circRNA Expression Vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10054)
AUTHORS Zhang Y, Xue W, Li X, Zhang J, Chen S, Zhang JL, Yang L, Chen LL
TITLE The Biogenesis of Nascent Circular RNAs.
JOURNAL Cell Rep. 2016 Apr 19;15(3):611-24. doi:
10.1016/j.celrep.2016.03.058. Epub 2016 Apr 7.
PUBMED 27068474
REFERENCE 2 (bases 1 to 10054)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10054
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2228..2541
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 2235..2253
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2271..2289
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2306..2324
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2342..2360
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2378..2396
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2413..2431
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2449..2467
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2514..2538
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2569..2578
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2620..2641)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 3942..3961
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 4289..4857
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 4307..4859
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4451..4468)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4678..4697
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind complement(5890..5909)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6237..6258
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 6444..7032
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6497..6517)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6915..6926)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7107..7340
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(7459..7477)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7545..7649
/gene="bla"
/label=AmpR promoter
CDS 7650..8510
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7868..7887)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8681..9269
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9170..9189
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9423..9440
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 9532..9551
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 9680..9701
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9716..9746
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9754..9770
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9759..9781
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9778..9794
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9778..9794
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9844..9863
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagttct
2221 tcactcaagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
2281 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
2341 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
2401 atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
2461 atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
2521 ttagtgaacc gtcagatcgc ctggagaaaa gcgctagcgc taccggtcgc caccatggtg
2581 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
2641 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
2701 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
2761 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
2821 gacttcttca agtccgccat gcccgaaggc tacgtccagg cacgtagcag gccagggcct
2881 ctctcagcca cctgagcaga aagctttcca agatagggca ggctgggtta ggccatctga
2941 gtctgtctcg ttcattggga tccagacttg actgtcttgt taaaggctgt tgctgcccag
3001 gtgtgcaggg agctgttggt ctctggcatt cagggtgggg gtggtataaa cccggggcag
3061 cttgcatatg gcagggaaga gggatccgtg gaggaacagt gcagaaggct ttatgttcag
3121 aatctctctt gcttttcttc tagactgagt tccttgagat tggtgaatgc tgtgtattat
3181 tcatccctga taacctggtg tttggcccag ggccttgtcc agaggagtgt ttgataagtg
3241 tttcaagtga attagcacca cgatgtcatc tcttttcagt ttacaaagga cggacacccc
3301 ctgacccggt ctcagaaagc ctgaaagcag aattagtcat tagaagggtg gttggcttgg
3361 tcggcataga ctttgagcag aaagaggttg aaaatgttga gcctgatttc tcttaggccc
3421 ctctgcagtg tctgttgtgg aggccagata cgtaactgct tccgcttttt ttggtgtcat
3481 tcaaggtgag caaatcccct tcatgtttct caccagacaa tgcagctgat gaggttccag
3541 ctttgcaaat gtagtcatcc atgaggactg tcttcctgag atttcatcag gctcgagtgg
3601 acttgcaaag gactttaggt ccattgtcct tttattctta gatacctctt tcactgagac
3661 cttttcctta cctcacctct ctaggtgacc aagggtggcc ccctgccctt cgcctgggac
3721 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
3781 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
3841 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
3901 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
3961 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
4021 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
4081 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
4141 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
4201 cgccactcca ccggcggcat ggacgagctg tacaaataag gtttagtgaa cggcgagatc
4261 aacttcagat cttgataact cgagcctctc cctccccccc cccctaacgt tactggccga
4321 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
4381 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
4441 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
4501 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
4561 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
4621 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
4681 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
4741 ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa
4801 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
4861 ggccacaacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
4921 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
4981 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtgt gtggtccaaa
5041 tggaaacctg gtgggcagtg gggctctggg gtgcaaggtg gaggctagag aggaagagct
5101 gtgttttttt tcctgactta cccagcagtg gtctgtgaga ttgtcttttc tggtgggcga
5161 acaaaaaggg ggttaggaaa actcaggcca aaaaagtgta aggcgttaat tccccattta
5221 attccttaaa atttcatgta ataccaggta ttgcctgtaa aggaaagata aagggaaaaa
5281 taagtaagac cttgttaaaa ttttattttt ctattttaac cttcacttat ttcctaatta
5341 ttaaaagaaa tttatgctta ttgttaagaa caaaaaaatt tcagtattac aatgaatttt
5401 taattaaaag tttttggcct gatgaaatct caggaagaca gtcctcatgg atgactacat
5461 ttgcaaagct ggaacctcat cagctgcatt gtctggtgag aaacatgaag gggatttgct
5521 caccttgaat gagaccaaaa aaagcggaag cagttacgta tctggcctcc acaacagaca
5581 ctgcagaggg gcctaagaga aatcaggctc aacattttca acctctttct gctcaaagtc
5641 tatgccgacc aagccaacca cccttctaat gactaattct gctttcaggc tttctgagac
5701 cgggtcaggg ggtgtcctta aaggttggaa aaaacttttc ctgtcatctt tgcctccaaa
5761 atctggcttt ctcccttggg cagggaaacc tccccaacat ttctctatca tccctgagat
5821 gtggggcctg cactctgact tctgtctgcc ttactctttg tcttacagga gcgcaccatc
5881 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
5941 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
6001 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
6061 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
6121 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
6181 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
6241 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
6301 taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
6361 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
6421 attgttgttg ttcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
6481 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
6541 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
6601 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
6661 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
6721 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
6781 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
6841 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
6901 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
6961 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
7021 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
7081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
7141 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
7201 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
7261 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
7321 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
7381 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
7441 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
7501 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
7561 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
7621 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
7681 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
7741 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
7801 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
7861 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
7921 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
7981 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
8041 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
8101 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
8161 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
8221 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
8281 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
8341 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
8401 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
8461 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
8521 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
8581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
8641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
8701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
8761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
8821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
8881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
8941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
9001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
9061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
9121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
9181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
9241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
9301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
9361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
9421 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
9481 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
9541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
9601 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
9661 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
9721 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
9781 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
9841 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
9901 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
9961 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
10021 gtatttgtta aatatgtact acaaacttag tagt
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：lentiSAM v2 (Puro)
- 下一篇：pDRGFP
